gene knock-out

The Study of Novel Mutation Detection Systems Based on Gene Knock-out Cells and Transgenic Mice

基因敲除细胞及转基因小鼠技术突变检测系统研究

The available models of gene knock-out mouse are classified and summarized to promote the development of related research .

本文对目前已获得的基因敲除鼠疾病模型进行了分类和总结，为相关研究的展开奠定了基础。

HPRT Gene Knock-out from Rat Fetal Neural Stem Cells

大鼠胎脑神经干细胞HPRT基因的敲除

The disease model of gene knock-out mouse is of significance in understanding the gene function and pathogenesis of human disease .

通过基因敲除建立的鼠疾病模型，在研究基因功能及人类疑难病症致病机制等方面发挥着前所未有的作用。

The same time , construction the process of gene knock-out mutants to explore optimization , master efficient , stable operation .

同时，在构建缺失突变体的过程中，经过摸索优化，掌握了高效、稳定的操作方法。

Establishment of a Hemophilia B Transgenic Mouse Model on the Basis of Coagulation Factor ⅸ Gene Knock-out Mouse

基于凝血因子Ⅸ基因剔除小鼠建立血友病乙转基因动物模型

Breeding , Reproducing , and Identifying for PAX-8 Gene Knock-out Mice

PAX-8基因敲除小鼠的饲养繁殖及基因型鉴定

Effects of high-fat diet on lipid metabolism in sr-a ⅰ / ⅱ gene knock-out mice

高脂膳食对SR-AⅠ/Ⅱ基因缺失小鼠脂代谢的影响

Construction of the sprE gene knock-out mutant of Enterococcus faecalis and its preliminary functional study

sprE基因敲除粪肠球菌突变株的构建和功能的初步研究

Generating Ornithine Decarboxylase Antizyme Inhibitor Gene Knock-out Mice by Trapping Vector

用基因捕获法制作鸟氨酸脱羧酶抗酶抑制子基因敲出小鼠

Effects of cyclin D1 gene knock-out on astrocyte proliferation and neuronal apoptosis in boundary zone following the focal cerebral ischemia

细胞周期蛋白D1基因敲除对小鼠脑缺血边缘区胶质细胞增殖及神经元凋亡的影响

Molecular and pathological characteristics of AS plaque formation induced by high-fat diet of LDLR and the APOE gene knock-out mice

高脂饮食诱导APOE和LDLR基因敲除鼠AS斑块形成的分子病理学特征

Objective : To establish an inducible model of glucocorticoid receptor ( GR ) gene knock-out mice using Cre / loxP system .

目的：应用Cre/loxP系统建立诱导型糖皮质激素受体（GR）基因剔除小鼠模型，为在体内直接进行GR基因的研究提供条件。

MAOB gene knock-out mice are resistant to the neurotoxin , 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine .

MAOB基因敲除小鼠对神经毒素1-甲基-4-苯基-1,2,3,6-四羟嘧啶（MPTP）具有耐受性。

Primary Study on Phenotype of Multi-organs Abnormality Resulting from PASG Gene Knock-out in C57BL / 6J Mice

C57BL/6J小鼠PASG基因敲除后多器官异常表型初探

The study and application of gene knock-out animal were reviewed in this article . Comparison between gene knock-out technique and RNA interference technique was also overviewed .

本文综述了基因敲除动物在各医学生物领域的研究与应用、浅谈其与RNA干扰技术的比较及其发展前景。

As a complement of gene knock-out , RNAi works as a simple , effective tool for promoting the study of functional genomics and gene therapy .

RNAi可作为一种简单有效的代替基因剔除的工具应用于功能基因组学和疾病的基因治疗研究。

Porcine α - 1 , 3-galactosyltransferase Gene Knock-out and HLA-G1 Gene Knock-in in Somatic Cells

猪体细胞α-1，3-半乳糖基转移酶基因的敲除并敲入HLA-G1基因的研究

Conclusion Cyclin D1 gene knock-out might partially inhibit the activation and decrease the proliferation of astrocyte and neuronal apoptosis after focal cerebral ischemia .

结论通过cyClinD1基因敲除，可部分抑制缺血边缘区胶质细胞增殖，同时减少神经元凋亡和脑梗死面积。该结果提示调控细胞周期可能是脑缺血新的治疗方向。

Construction and Characteristic Analysis for the hrpX / hrpG Gene Knock-out Mutant of Xanthomonas Oryzae Pv . oryzae

白叶枯病菌hrpX/hrpG双基因敲除突变体的构建及特性研究

With the development of mouse genetic engineering technology , the application of gene knock-out mouse model and transgenic mouse model to study the relationship between gene and human diseases is becoming the hot-spot of research .

随着小鼠遗传工程技术的发展，利用基因敲除、转基因小鼠模型来研究基因与人类疾病的关系，已成为人们研究的热点。

Conclusion Connexin 43 gene knock-out might partially inhibit the analgesic effect of acupuncture , suggesting that connexin 43 is possibly related with meridians and the effect of acupuncture .

结论：敲除缝隙连接蛋白Cx43基因可部分抑制针刺镇痛效应，提示Cx43与经络及针刺镇痛效应具有一定的相关性。

This new technology is now used to research gene knock-out , signal pathway , gene regulation and gene therapy . Compared to those methods inducing gene silence , RNAi is more specific and more effective .

目前RNAi已应用于基因敲除、基因表达调控、信号转导和基因治疗等方面的研究，与以往基因沉默的研究方法相比，RNAi能够更加高效特异地进行特定基因的抑制。

Conclussion : We have obtained C57BL / 6J ES cell lines with a potential germ-line contribution , which can be applied to generate transgenic and gene knock-out mice .

结论：建立了能实现种系传递的C57BL/6J小鼠胚胎干细胞系，该系的ES细胞可用于今后制备转基因动物和基因敲除动物。

Foundation of gene knock-out mice is a complex systematic project , and this study perfected and normalized the key technique steps of the technology system of gene knock-out mice . To optimize the basic cultivation conditions of ES cells ;

基因剔除小鼠的建立是一项复杂的系统工程，本研究对基因剔除小鼠技术体系的关键技术环节进行了完善和规范：优化了ES细胞的基本培养条件；

Scientists have tried to overcome myelin inhibition by delivering antibody IN-1 , Nogo gene knock-out , applying Nogo antagonists and so on . But unfortunately , they did not get satisfied results .

为克服髓鞘抑制分子的作用，研究者们尝试了多种不同的方法，如，使用抗Nogo-A抗体IN-1，敲除Nogo基因和使用Nogo分子拮抗剂等，但是都没有取得非常令人满意的效果。

Based on the homologous recombination , the plasmid pRG of reverse gyrase gene knock-out was constructed and transformed into the Sulfolobus islandicus and the single crossover mutant was isolated .

重组质粒反复转化冰岛硫化叶菌，得到质粒整合到宿主染色体上的单交换菌株，但没有获得反螺旋酶基因缺失的双交换转化子。

Effect of Bone Marrow Stem Cells Transplantation on Microstructure of Skeleton Muscle in Dystrophin / utrophin Gene Double Knock-out Mice

骨髓干细胞移植对dystrophin/utrophin基因双敲除鼠骨骼肌微观结构的影响

Objective Study the improvement of locomotive faculty of dystrophin / utrophin gene double knock-out mice ( dko mice ) by transplanting bone marrow stem cells .

目的研究骨髓干细胞移植治疗Duchenne型肌营养不良鼠（dystrophin/utrophingenedoubleknock-outmice，dko鼠）的运动功能改善情况。

Insertion mutation can be applied to this research , but it is limited by gene randomness , lethal knock-out , non tagged mutants .

对于这方面的研究，插入突变技术就是一个可用的工具，但是基因的随机性，致死敲除，无标记的突变体都大大地限制了这种技术的利用。